BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
PCT（降钙素原，procalcitonin）是一种蛋白质， 它由116个氨基酸组成，由甲状腺滤泡旁细胞（C细胞）以及肺和肠道的神经内分泌细胞产生。降钙素水平在对促炎症刺激的反应中上升，尤其是细菌引起的刺激。降钙素的诱导期从4-12小时不等，半衰期从22-35小时不等。随着严重感染带来的炎症级联和全身反应，降钙素的血液水平可能上升多个数量级，更高的数值与更严重的疾病相关。然而，感染期间产生的高降钙素水平并不伴随着降钙素的平行增加或血清钙水平的下降。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠降钙素原(PCT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠降钙素原(PCT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse PCT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse PCT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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