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高分子量脂联素（HMW Adiponectin）是脂联素的一种高聚合形态，主要由脂肪细胞分泌，在血浆中以多聚体形式存在。作为脂联素亚型中生物活性最强的形式，HMW Adiponectin在调节糖脂代谢、抗炎及心血管保护中发挥关键作用。HMW Adiponectin通过激活AMPK通路增强胰岛素敏感性，促进葡萄糖摄取和脂肪酸氧化，从而改善代谢综合征相关疾病。其水平与肥胖、胰岛素抵抗及2型糖尿病呈负相关，且在预测心血管疾病风险中具有重要价值。临床研究显示，HMW Adiponectin相较于总脂联素更能反映代谢健康状态，是评估胰岛素抵抗和心血管风险的独立指标。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠高分子量脂联素(HMWAdiponectin)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠高分子量脂联素(HMWAdiponectin)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse HMWAdiponectin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse HMWAdiponectin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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