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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
5-羟色胺受体4是一种蛋白质,是由HTR4基因编码的。该基因是5-羟色胺受体家族中的一员,是G蛋白偶联受体,对5-羟色胺的反应刺激cAMP的产生。该基因产物是一种糖基化的跨膜蛋白,在外周和中枢神经系统中发挥着调节各种神经递质释放的功能。编码具有不同C端序列的蛋白质的多种转录变体已被描述,但一些转录变体的全长性质尚未确定。该受体位于消化道、膀胱、心脏和肾上腺以及中枢神经系统(CNS)。在中枢神经系统中,该受体出现在putamen、caudate nucleus、nucleus accumbens、globus pallidus和substantia nigra,在较小程度上出现在新皮质、raphe、pontine nuclei和丘脑的一些区域。在小脑中还没有发现这种情况。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人5-羟色胺受体4(HTR4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人5-羟色胺受体4(HTR4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HTR4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HTR4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.