计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
5-羟色胺受体6(HTR6)是5HT受体的一个亚型,与内源性神经递质5-羟色胺(5-hydroxytryptamine,5HT)结合。它是一个G蛋白偶联受体(GPCR),与Gs偶联,介导兴奋性神经传递。该受体几乎只在大脑中表达。它分布在不同的区域,包括但不限于嗅结节、大脑皮层(额叶和内侧区)、阿肯色核、纹状体、尾状核、海马和小脑分子层。根据其在锥体外系、边缘系和皮质区的丰富程度,可以认为它在运动控制、情感、认知和记忆等功能中发挥着作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人5-羟色胺受体6(HTR6)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人5-羟色胺受体6(HTR6)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HTR6. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HTR6 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.