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8-羟基鸟嘌呤DNA糖苷酶（hOGG1）是一种DNA糖基化酶，由OGG1基因编码。它是负责切除8-氧代鸟嘌呤（8-oxoG）的主要酶，8-氧代鸟嘌呤是暴露于活性氧（ROS）而产生的诱变碱基副产物。OGG1是一种双功能的糖基化酶，因为它既能裂解诱变病变的糖苷键，又能导致DNA骨架上的断链。该基因C端区域的替代剪接将剪接变体分为两大类，1型和2型，取决于序列的最后一个外显子。1型替代剪接变体以第7外显子为终点，2型以第8外显子为终点。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人8-羟基鸟嘌呤DNA糖苷酶(hOGG1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人8-羟基鸟嘌呤DNA糖苷酶(hOGG1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human hOGG1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human hOGG1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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