BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ATP酶蛋白酶体26S亚基3(PSMC3)也被称为26S蛋白酶体AAA-ATP酶亚单位Rpt5，是一种由PSMC3基因编码的酶。该蛋白是完整组装的19S蛋白体复合物的19个基本亚单位之一，六个26S蛋白体AAA-ATP酶亚单位（Rpt1、Rpt2、Rpt3、Rpt4、Rpt5（该蛋白）和Rpt6）与四个非ATP酶亚单位（Rpn1、Rpn2、Rpn10和Rpn13）一起构成蛋白体复合物19S调节颗粒的基础亚复合物。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人ATP酶蛋白酶体26S亚基3(PSMC3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人ATP酶蛋白酶体26S亚基3(PSMC3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PSMC3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PSMC3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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