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A激酶PRKA锚定蛋白12(Akap12)是一种由AKAP12基因编码的酶。A-激酶锚定蛋白(AKAPs)是一组结构不同的蛋白,它们的共同功能是与蛋白激酶A(PKA)的调节亚单位结合,并将全酶限制在细胞内的不同位置。该基因编码AKAP家族的一个成员。编码的蛋白在内皮细胞、培养的成纤维细胞和骨肉瘤细胞中表达。它与蛋白激酶A和C以及磷酸酶相关联,在信号转导中作为一种支架蛋白。该蛋白和RII PKA在细胞周边共定位。该蛋白是一种与细胞生长有关的蛋白。重症肌无力患者可产生该蛋白的抗体。该基因的替代剪接导致两个转录变体,编码不同的异构体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人A激酶PRKA锚定蛋白12(Akap 12)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人A激酶PRKA锚定蛋白12(Akap 12)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human Akap 12. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human Akap 12 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.