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Bcl2 相关髓细胞白血病序列 1,也被称为 MCL1,是由 MCL1 基因编码的蛋 白质。该基因编码的蛋白质属于 Bcl-2 家族。可变剪接发生在该基因座处, 并且已鉴定出编码不同同种型的两种转录变体。较长的基因产物(同种型 1) 通过抑制细胞凋亡来提高细胞存活率,而交替剪接的较短基因产物(同种型 2)促进细胞凋亡并诱导死亡。蛋白质 MCL1 的生物半衰期非常短,仅为 20-30 分钟。它的丢失比 Bcl-2 家族的任何其他抗凋亡成员的丢失具有更大 的影响。当胚胎只有大约 3.5 天大时,甚至在植入之前,该基因的缺失会导 致胚胎死亡。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人Bcl2相关髓细胞白血病序列1(MCL1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Bcl2相关髓细胞白血病序列1(MCL1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MCL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MCL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.