BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
C-型凝集素域家族11成员A，也被称为CLEC11A，是由CLEC11A基因编码的。该基因编码C型凝集素超家族的成员。编码的蛋白质是分泌的硫酸化糖蛋白，并作为原始造血祖细胞的生长因子。已经描述了一种替代的剪接变体，但其生物学性质尚未确定。与它相关的疾病包括慢性肉芽肿病和先天性肌无力综合征7。其相关途径包括CREB途径和ERK信号传导。与该基因相关的基因本体注释包括碳水化合物结合和生长因子活性。该基因的一个重要副基因是CLEC3B。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人C-型凝集素域家族11成员A(CLEC11A)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人C-型凝集素域家族11成员A(CLEC11A)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CLEC11A. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CLEC11A present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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