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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
C1q 肿瘤坏死因子相关蛋白 1,也被称为 CTRP1,是由 C1QTNF1 基因编码 的蛋白质。启用胶原蛋白结合活性。参与多个过程,包括血小板聚集的负调 节;醛固酮分泌的正向调节;和细胞溶质钙离子浓度的正向调节。位于细胞 外空间。是质膜的组成部分。C1q/TNF 相关蛋白 1 (CTRP1) 是脂联素的旁系同源物,属于 CTRP 亚家族。CTRP1 在脂肪细胞、巨噬细胞、心肌细胞和其他细胞中表达和分泌。它促进脂质和葡萄糖代谢,但对炎症的调节具有双向作用。炎症也会反向刺激 CTRP1 的产生。两者之间可能存在恶性循环。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人C1q肿瘤坏死因子相关蛋白1(CTRP1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人C1q肿瘤坏死因子相关蛋白1(CTRP1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CTRP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CTRP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.