计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
C1q 和肿瘤坏死因子相关蛋白 4 是一种蛋白质,由 C1QTNF4 基因编码。Cq1TNF4,也被称为 CTRP4,是一种分泌蛋白,是 Acrp30/Adiponectin 的至少 9 个类似物之一。这个家族的蛋白质含有一个球状的 C 端结构域,与C1q 和 TNF 配体家族的结构域相似。C1qTNF4 是唯一缺乏胶原蛋白样结构域的家族成员,但有两个 C1q 结构域。成熟的人 C1qTNF4 与小鼠 C1qTNF4的相应区域有 92.4%的氨基酸序列同源性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人C1q肿瘤坏死因子相关蛋白4(CTRP4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人C1q肿瘤坏死因子相关蛋白4(CTRP4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CTRP4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CTRP4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.