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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CD73 分子(CD73)又称 5′-核苷酸酶(5′-NT)或外生-5′-核苷酸酶,是一种表面酶,在多种细胞上表达。这种酶介导 ATP 和 ADP 的自分泌和旁分泌危险信号逐渐水解为抗炎的腺苷。由腺苷途径介导的免疫抑制对于维持免疫系统的平衡非常重要。T 调节细胞的免疫抑制功能也依赖于 CD73 的表达。Treg 一般会抑制免疫反应。它们影响T 细胞的增殖和功能。它也出现在过敏性CD4+ T 细胞上,从而维持对健康组织的自我耐受,以及在怀孕期间保护胎儿免受母亲免疫系统的影响。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人CD73分子(CD73)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人CD73分子(CD73)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CD73. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CD73 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.