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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Cullin 9蛋白(CUL9),也被称为p53相关的parkin样胞质蛋白或UbcH7相关蛋白1,是由CUL9基因编码的蛋白质。它是E3泛素连接酶最大家族(称为库林环连接酶(CRL))的非规范且特征不良的成员。大多数CRL在发育过程中起着关键作用,然而,CUL9在神经元发育中的作用仍然难以捉摸。它被认为是一种独特的CRL,因为它在其C端的RING(RBR)结构域之间包含一个额外的RING,并且没有确定的适配器蛋白。Cul9-RING泛素蛋白连接酶复合物的核心组分,该复合物介导泛素化和随后的BIRC5降解,并且是维持微管动力学和基因组完整性所必需的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人Cullin 9蛋白(CUL9)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Cullin 9蛋白(CUL9)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CUL9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CUL9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.