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C型凝集素域家族4成员A(DCIR)是一种蛋白质,是由DCIR基因编码的。该基因编码的是C型凝集素/C型凝集素样结构(CTL/CTLD)超家族的成员。这个家族的成员有一个共同的蛋白折叠,具有不同的功能,如细胞粘附、细胞-细胞信号、糖蛋白周转以及在炎症和免疫反应中的作用。编码的2型跨膜蛋白可能在炎症和免疫反应中起作用。该基因的多个转录变体编码不同的异构体已被确认。该基因与染色体12p13上的其他CTL/CTLD超家族成员在自然杀伤基因复合物区域紧密相连。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人C型凝集素域家族4成员A(DCIR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人C型凝集素域家族4成员A(DCIR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human DCIR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human DCIR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.