BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Dectin-2蛋白(Dectin-2)是由Dectin-2编码的蛋白，该基因所编码的蛋白是一种II型膜受体，具有细胞外C型凝集素样结构域的折叠。胞外部分结合具有高甘露糖含量的结构，并已被证明能识别几种病原体，包括优雅氏菌、脑葡萄球菌、结核杆菌、新形虫和屋尘螨。当受到刺激时，编码的蛋白通过CARD9-Bcl10-Malt1途径启动信号传导，导致细胞因子的诱导。它也是一种检测真菌细胞壁成分的PRR。它能识别α-甘露聚糖，因此能够检测真菌。与它相关的疾病包括隐球菌病和皮下真菌病。其相关途径包括CLEC7A（Dectin-1）信号传导和C1GALT1C1缺陷导致Tn聚凝血综合征（TNPS）。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Dectin-2蛋白(Dectin-2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Dectin-2蛋白(Dectin-2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human Dectin-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human Dectin-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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