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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Exostosin1（EXT1）是一种由EXT1基因编码的Ⅱ型跨膜糖基转移酶，广泛分布于内质网，参与硫酸乙酰肝素（HS）生物合成的链延长过程。作为外泌素糖基转移酶家族成员，EXT1在细胞外基质（ECM）合成与修饰中发挥关键作用，尤其在多糖链的延长和硫酸化过程中至关重要。EXT1的功能异常与多种疾病相关。其基因突变会导致多发性骨病Ⅰ型，表现为骨骼发育异常；在肿瘤领域，EXT1表达异常与遗传性多发性骨软骨瘤、胆囊癌及鳞状细胞癌等恶性转化密切相关。此外，EXT1还参与炎症反应，如慢性根尖周炎的病理过程，并可能在狼疮膜性肾病中作为潜在生物标志物。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Exostosin1(EXT1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Exostosin1(EXT1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human EXT1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human EXT1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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