BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
G补缀FHA域血管生成因子1(AGGF1)是一种蛋白质，由AGGF1基因编码。 该基因是具有G补丁和叉头相关结构域的血管生成因子。它主要在激活的、丰满的内皮细胞中表达，起到调节血管生成和血管发育的作用。AGGF1与参与血管发育的多种蛋白质相互作用。它的突变与多种癌症有关，可导致罕见的先天性疾病--克里佩尔-特雷瑙内综合症。虽然AGGF1主要在内皮细胞、血管平滑肌细胞和成骨细胞中发挥作用，但它在肥大细胞、心脏细胞、Kupffer细胞和造血干细胞中也具有活性。在心脏、肾脏和四肢中检测到AGGF1 mRNA，这表明该蛋白很可能也在这些器官中发挥作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人G补缀FHA域血管生成因子1(AGGF1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人G补缀FHA域血管生成因子1(AGGF1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human AGGF1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human AGGF1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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