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Janus激酶（JAK）是一个细胞内的非受体酪氨酸激酶家族，通过JAK-STAT途径转发细胞因子介导的信号。它们最初被命名为"另一种激酶"1和2（因为它们只是在基于PCR的激酶筛选中发现的两个），但最终被命名为"Janus激酶"。JAKs拥有两个几乎相同的磷酸盐转移结构域。一个结构域表现出激酶活性，而另一个结构域对第一个结构域的激酶活性进行负向调节。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Janus激酶(JAK)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Janus激酶(JAK)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human JAK. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human JAK present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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