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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
骨桥蛋白(osteopontin, OPN)不仅是一种分泌型基质细胞细胞因子,还是一种循环促炎细胞因子,可诱导细胞粘附、迁移和炎症途径的激活。OPN 表达的慢性增加与主要不良心血管事件的风险增加有关。在炎症细胞浸润过程中,OPN 可在 168R/S169 位点被凝血酶裂解成两个片段,其中一个是 OPN 的 N 端片段(N-OPN),它暴露了一个隐蔽的整合素结合基序 162SVVYGLR168(小鼠中的 SLAYGLR),并与 α4β1 和 α9β1 整合素结合,从而发挥强大的促炎作用。OPN-N 通过增加白细胞介素 (IL)-17 和 IL-6 的产生在多发性硬化症中起关键作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人N末端骨桥蛋白(N-Osteopontin)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人N末端骨桥蛋白(N-Osteopontin)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human N-Osteopontin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human N-Osteopontin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.