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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
N端前脑钠素（NT-proBNP或BNPT）是一种具有76个氨基酸的N端非活性蛋白的前体，从分子中裂解出来的是脑钠肽。血液中的BNP和NT-proBNP水平均可用于筛查、诊断急性充血性心力衰竭（CHF），并可用于确定心力衰竭的预后，因为这两种标志物通常在预后较差的患者中较高。无症状或有症状的左心室功能障碍患者的血浆浓度通常也会增加，并与冠状动脉疾病和心肌缺血有关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人N端前脑钠素(NT-ProBNP)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人N端前脑钠素(NT-ProBNP)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NT-ProBNP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NT-ProBNP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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