BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
p53诱导基因3（PIG3），又叫肿瘤蛋白p53诱导蛋白3（TP53IG3），是由PIG3基因编码的酶。该基因编码的蛋白质类似于氧化还原酶，氧化还原酶是参与细胞对氧化应激和辐射作出反应的酶。该基因由肿瘤抑制基因p53诱导，被认为与p53介导的细胞死亡有关。它在其启动子区域包含一个p53共有结合位点和一个下游的五核苷酸微卫星序列。P53已被证明通过与下游五核苷酸微卫星序列相互作用来转录激活该基因。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人p53诱导基因3(PIG3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人p53诱导基因3(PIG3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PIG3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PIG3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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