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S100钙结合蛋白A12(S100A12)是由S100A12基因编码的蛋白质。该基因所编码的蛋白是S100家族中的一员,含有2个EF手的钙结合基团。一般来说,S100A12具有显著的抗感染和抗菌作用,这与它吸收离子的能力有关。中性粒细胞和单核细胞/巨噬细胞是细胞中S100A12的重要来源,尽管一些上皮细胞和树突状细胞也能分泌。一些组织富含这些细胞,因此也富含这种蛋白,这些组织包括脾脏或肺部。它发生在细胞内,但也产生到细胞外环境中,在那里它发挥着主要作用。它在细胞外环境中以同二聚体或六聚体的形式出现。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人S100A12钙结合蛋白(S100A12)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人S100A12钙结合蛋白(S100A12)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human S100A12. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human S100A12 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.