计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
S100钙结合蛋白A10(S100A10),又称p11,是一种蛋白质,在人类中由S100A10基因编码,在其他物种中由S100a10基因编码。S100A10是S100蛋白家族的成员,含有两个EF手钙结合图案。S100蛋白定位于多种细胞的细胞质和/或细胞核中。它们调节一些细胞过程,如细胞周期的进展和分化。S100蛋白通过F-actin的重组与细胞外渗和内渗有关。p11蛋白可以以自由单体、同源二聚体或由p11二聚体与两分子附件素II组成的异源四聚体的形式出现。同源二聚体或异源四聚体又可以通过形成两个二硫键而二聚。p11单体是一个不对称的蛋白质,由四个α螺旋组成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人S100钙结合蛋白A10(S100A10;P11)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人S100钙结合蛋白A10(S100A10;P11)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human S100A10;P11. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human S100A10;P11 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.