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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
T-框蛋白3(TBX3),也被称为T-box转录因子TBX3,是由TBX3基因编码的蛋白质。它是转录因子的T-box基因家族的成员,它们都共享一个称为T-box的高度保守的DNA结合域。T-box基因家族由17个成员组成,分为五个亚家族,即Brachyury,T-brain,TBX1,TBX2和TBX6。它具有对其转录因子功能很重要的结构域,其中包括一个也称为T盒的DNA结合结构域(DBD),一个核定位信号,两个抑制结构域(R2和R1)和一个激活结构域(A)。它可以通过结合T元件或半个T元件位点来激活和/或抑制其靶基因。它作为转录抑制因子或转录激活剂的功能部分受到蛋白质辅助因子的调节。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人T-框蛋白3(TBX3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人T-框蛋白3(TBX3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TBX3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TBX3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.