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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Tafazzin蛋白是一种蛋白质,由TAFAZZIN基因编码。Tafazzin在心脏和骨骼肌中高度表达,并作为一种磷脂-糖磷脂转酰酶(它属于磷脂:二酰甘油酰基转移酶)发挥作用。它催化未成熟的心磷脂重塑为成熟的成分,其中主要是四氢油酰基。TAFAZZIN基因产生几种不同的tafazzin蛋白异构体。每个异构体都有一个长的形式和一个短的形式;短的形式缺乏一个疏水的领导序列,可能作为一个细胞质蛋白而不是膜结合蛋白存在。其他交替剪接的转录本已被描述。大多数异构体在所有组织中都有,但有些只在某些类型的细胞中发现。TAFAZZIN基因位于X染色体q臂的第28位,它跨越了10,208个碱基对。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人Tafazzin蛋白(TAZ)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Tafazzin蛋白(TAZ)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TAZ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TAZ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.