BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Visinin 样蛋白 1 由 VSNL1 基因编码的蛋白。该基因是神经元钙传感器蛋白的 visinin / recoverin 亚家族的成员。编码的蛋白质在小脑的颗粒细胞中强烈表达，在小脑的颗粒细胞中，其以钙依赖性方式与膜结合，并通过直接或间接调节腺苷酸环化酶的活性来调节中枢神经系统的细胞内信号传导途径。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Visinin样蛋白1(VILIP-1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Visinin样蛋白1(VILIP-1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human VILIP-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human VILIP-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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