BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α1 抗胰糜蛋白酶(AACT)是一种α球蛋白糖蛋白是丝氨酸超家族的成员。它 是由 SERPINA3 基因编码的。它通过将蛋白酶裂解成不同的形状或构象来抑 制某些被称为蛋白酶的酶的活性，如在中性粒细胞中发现的蛋白酶 G 和在肥大细胞中发现的糜烂酶。这种活动可以保护一些组织，如下呼吸道免受蛋白酶的损害。它在肝脏中产生，是炎症期间诱发的一种急性期蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人α1抗胰糜蛋白酶(AACT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人α1抗胰糜蛋白酶(AACT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human AACT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human AACT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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