BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-防御素 3 是一种蛋白质，由 DEFB3 基因编码。RT-PCR 显示 HBD-3 在皮 肤，气管，舌头和扁桃体中高表达，而在唾液腺，子宫，肾脏，骨髓，胸腺， 结肠，胃，腺样体，咽和喉中表达水平较低。防御素是抗菌肽的一种，其中 β-防御素 3(HBD-3)主要分布于皮肤和粘膜的上皮细胞中，具有广谱的抗菌 活性，尤其是对包括金黄色葡萄球菌在内的革兰阳性菌具有强烈的杀伤作用， 构成抵御病菌侵袭的一道有力的化学屏障；同时还发现，hBD3 具有重要的 免疫调节作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人防御素β3(DEFβ3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人防御素β3(DEFβ3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human DEFβ3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human DEFβ3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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