计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
γ-干扰素诱导蛋白 IFI-16(IFI-16)也被称为干扰素诱导的骨髓分化转录激活剂,是一种由 IFI16 基因编码的蛋白质。该基因编码的是 HIN-200(具有200 个氨基酸重复的造血干扰素诱导的核抗原)细胞因子家族的成员。编码的蛋白含有参与 DNA 结合、转录调节和蛋白-蛋白相互作用的结构域。该蛋白定位于核质和核小体,并与 p53、retinoblastoma-1 和 BRCA1 相互作用。它调节 p53 功能,并在 Ras/Raf 信号通路中抑制细胞生长。IFI16 已被证明在感知细胞内 DNA 方面发挥作用--这是病毒感染细胞的一个标志。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人γ干扰素诱导蛋白16(IFI16;p16)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人γ干扰素诱导蛋白16(IFI16;p16)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IFI16;p16. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IFI16;p16 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.