BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ⅴ型胶原α2(COL5A2)是一种蛋白质，由COL5A2基因编码。该基因编码的是低丰度纤维状胶原蛋白之一的α链。纤维状胶原蛋白分子是三聚体，可以由一个或多个类型的α链组成。V型胶原蛋白存在于含有I型胶原蛋白的组织中，并且似乎可以调节由I型和V型胶原蛋白组成的异型纤维的组装。该基因产物与XI型胶原蛋白密切相关，可能V型和XI型的胶原蛋白链构成一个单一的胶原蛋白类型，具有组织特异性的链组合。该基因的突变与Ehlers-Danlos综合征有关，经典类型，以前称为I型和II型。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Ⅴ型胶原α2(COL5α2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Ⅴ型胶原α2(COL5α2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human COL5α2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human COL5α2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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