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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
NOSTRIN是一个蛋白编码基因。与NOSTRIN相关的疾病包括子痫和子痫前期。其相关途径包括eNOS的激活和调节以及新陈代谢。该基因的一个重要同源物是CD2AP。NOSTRIN包含一条506-aa残基的单多肽链，58kDa，有一个N端cdc15结构域和一个C端SH3结构域。NOSTRIN mRNA在高度血管化的组织如胎盘、肾脏、肺和心脏中含量丰富，NOSTRIN蛋白在血管内皮细胞中表达。一氧化氮（NO）是生物过程中强有力的媒介，如神经传递、炎症反应和血管稳态。NOSTRIN与负责产生NO的酶--内皮NO合酶结合，并触发ENOS从质膜向囊泡状亚细胞结构的转移，从而减弱ENOS依赖的NO产生。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人一氧化氮合酶运输因子(NOSTRIN)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人一氧化氮合酶运输因子(NOSTRIN)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NOSTRIN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NOSTRIN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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