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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
三叶因子3,也被称为TFF3,它是由TFF3基因编码的蛋白质,是三叶因子家族的成员,它们的特征在于具有至少一个三叶因子基序的副本,三叶因子基序是一个40-氨基酸结构域,含有三种保守的二硫化物。三叶草因子(TFF)是产生粘蛋白的细胞的分泌产物。它们在维持口腔粘膜表面完整性和通过称为恢复的过程增强胃肠粘膜愈合方面起着关键作用。它们在胃肠道内的上皮恢复中具有重要和必要的作用。它们是在胃肠粘膜中表达的稳定分泌蛋白。它们的功能多种多样,可以保护粘膜,稳定粘液层并增加上皮的愈合率。该基因是柱状上皮的标志物,在各种组织中表达,包括肠道和结肠的杯状细胞。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人三叶因子3(TFF3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人三叶因子3(TFF3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TFF3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TFF3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.