BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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上皮细胞粘附分子（EpCAM）是一种跨膜糖蛋白，在上皮细胞中介导Ca2+无关的同型细胞-细胞粘附。它还参与细胞信号传递、迁移、 增殖和分化。 此外，它具有致癌潜力，它能够上调c-myc、e-fabp和细胞周期蛋白A和E。由于EpCAM只在上皮细胞和上皮细胞衍生的肿瘤中表达，它可以作为各种癌症的诊断标志物。它在肿瘤的发生和转移中起作用，所以它也可以作为一个潜在的预后标记，并作为免疫治疗策略的潜在目标。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人上皮细胞黏附分子(EPCAM)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人上皮细胞黏附分子(EPCAM)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human EPCAM. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human EPCAM present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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