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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丝甘蛋白聚糖,又称造血蛋白多糖核心蛋白或分泌颗粒蛋白多糖核心蛋白,是一种由 SRGN 基因编码的蛋白质。它主要在造血细胞和内皮细胞中表达,是唯一已知的细胞内蛋白多糖。该基因编码的蛋白质最著名的是造血细胞颗粒蛋白多糖。储存在许多造血细胞的分泌颗粒中的蛋白多糖还含有一个抗蛋白酶的肽核,这可能对中和水解酶很重要。该编码蛋白被发现与颗粒酶和穿孔蛋白的大分子复合物有关,它可能作为颗粒介导的细胞凋亡的媒介。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人丝甘蛋白聚糖(SRGN)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人丝甘蛋白聚糖(SRGN)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SRGN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SRGN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.