BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丫叉同源物3（SSH3），也被称为蛋白磷酸酶同源物弹弓3，是由SSH3基因编码的酶，属于磷酸酶的Slingshot同源物（SSH）家族。ADF（肌动蛋白解聚因子）/共纤素家族由肌动蛋白动力学的刺激响应介质组成。ADF/cofilin蛋白被激酶如LIM结构域激酶-1灭活。SSH家族似乎通过在体内重新激活ADF/cofilin蛋白在肌动蛋白动力学中发挥作用。它在血管内肿瘤血栓和淋巴结转移中被上调。它可能是肿瘤启动子和CRC进展的预后标志物。SSH3、LIMK1和Rac1的功能同调可能导致细胞骨架的快速周转，并最终促进CRC的侵袭和转移。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人丫叉同源物3(SSH3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人丫叉同源物3(SSH3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SSH3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SSH3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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