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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
中期因子(MK)又称神经元生长促进因子2(NEGF2),是一种由MDK基因编码的蛋白质。它是一种低分子量的基本肝素结合的生长因子,与pleiotrophin(NEGF1,与MK同源46%)形成一个家族。它是一个非糖基化的蛋白质,由两个由二硫桥连接的结构域组成。它是一种重要的发展性视黄酸反应基因产物,在妊娠中期被强烈诱导,因此被称为midkine。它具有多效性,能够发挥诸如细胞增殖、细胞迁移、血管生成和纤维蛋白溶解等活动。一个包含受体型酪氨酸磷酸酶zeta(PTPζ)、低密度脂蛋白受体相关蛋白(LRP1)、无色素白血病激酶(ALK)和syndecans的分子复合物被认为是其受体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人中期因子(MK)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人中期因子(MK)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MK. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MK present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.