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醛脱氢酶 1 家族成员 A2,也被称为 ALDH1A2 或 ALDH1A2 或视黄醛脱氢酶 2(RALDH2),是由 ALDH1A2 基因编码的酶。这种蛋白质属于醛脱氢酶家族的蛋白质。该基因的产物是一种催化视黄酸(RA)从视黄醛合成的酶。视黄酸是维生素 A(视黄醇)的活性衍生物,是一种旁分泌激素信号分子,在发育和成年组织中起作用。对类似小鼠基因的研究表明,这种酶和细胞色素 CYP26A1 同时建立了局部胚胎视黄酸水平,促进后器官发育并预防脊柱裂。已经为该基因鉴定出三个编码不同亚型的转录本变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人乙醛脱氢酶1家族成员A2(ALDH1A2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人乙醛脱氢酶1家族成员A2(ALDH1A2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ALDH1A2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ALDH1A2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.