BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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乙醛脱氢酶1家族A3成员，又称ALDH1A3或视黄醛脱氢酶3（RALDH3），是由ALDH1A3基因编码的酶。醛脱氢酶同工酶被认为在酒精代谢和脂质过氧化产生的醛的解毒中起着重要作用。该基因所编码的酶以视黄醇为底物，以游离的或细胞视黄醇结合蛋白的形式出现。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人乙醛脱氢酶家族1-A3(ALDH1A3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人乙醛脱氢酶家族1-A3(ALDH1A3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ALDH1A3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ALDH1A3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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