BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Semaphorin 7A, 信号素7A（SEMA7A）也被称为CD108（Cluster of Differentiation 108）。SEMA7A是一种膜结合的信号素，通过糖基磷脂酰肌醇（GPI）连接与细胞表面结合。SEMA7A也被称为约翰-米尔顿-哈根（JMH）血型抗原，是一种在活化淋巴细胞和红细胞上表达的80kD糖蛋白。它在各种成人组织中表达，如脂肪、结肠、食管、心脏、大脑、脾脏、睾丸、肺、卵巢和子宫。SEMA7A促进轴突生长并参与中胚层衍生的体细胞形成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人信号素7A(SEMA7A)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人信号素7A(SEMA7A)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SEMA7A. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SEMA7A present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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