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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
信号调节蛋白 α (SIRPα) 是一种来自 SIRP 家族的调节膜糖蛋白,主要由 骨髓细胞以及干细胞或神经元表达。它充当抑制性受体并与广泛表达的跨膜 蛋白 CD47 相互作用,也称为“不要吃我”信号。这种相互作用消极地控制 先天免疫细胞的效应功能,例如宿主细胞的吞噬作用。它在巨噬细胞膜上横 向扩散并在吞噬突触处积聚以结合 CD47 并发出“自我”信号,从而抑制巨 噬细胞吞噬细胞骨架的密集过程。这类似于 MHC I 类分子通过 Ig 样或 Ly49 受体向 NK 细胞提供的自身信号。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人信号调节蛋白α(SIRPα)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人信号调节蛋白α(SIRPα)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SIRPα. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SIRPα present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.