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内分泌腺来源血管内皮生长因子(EG-VEGF)是一种组织特异性的血管生成因子,是prokineticin家族的成员。它主要局限于类固醇生成的腺体,即卵巢、睾丸、肾上腺和胎盘。此外,它能促进肾上腺衍生的微血管内皮细胞的增殖、迁移和分化,但不能促进其他(如人脐静脉内皮细胞)。当输送到卵巢或睾丸时,它也能促进血管生成,但不是其他组织。EG-VEGF在人类发育早期就有表达,而在成人中,EG-VEGF只在Leydig细胞和Leydig细胞肿瘤中强烈表达,而不在其他睾丸肿瘤(如生殖细胞衍生的肿瘤)中表达。EG-VEGF由缺氧诱导,该基因的启动子上有一个HIF结合点。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人内分泌腺来源血管内皮生长因子(EG-VEGF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人内分泌腺来源血管内皮生长因子(EG-VEGF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human EG-VEGF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human EG-VEGF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.