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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
内皮糖蛋白(ENG)是一种位于细胞表面的I型膜糖蛋白,是TGF beta受体复合物的一部分。它通常也被称为CD105、END、FLJ41744、HHT1、ORW和ORW1。它在血管生成中具有关键作用,因此,它是肿瘤生长、生存和癌细胞转移到身体其他位置的重要蛋白。人类其基因位于人类9号染色体上,其细胞生成带的位置在9q34.11。该糖蛋白由180kDA的同源二聚体组成,通过分子间的二硫键稳定。它有一个约561个氨基酸的大胞外结构域、一个疏水跨膜结构域和一个由45个氨基酸组成的短胞质尾部结构域。最接近细胞外膜的260个氨基酸区域被称为ZP结构域。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人内皮糖蛋白(ENG)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人内皮糖蛋白(ENG)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ENG. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ENG present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.