BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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FAS又称Fas受体、FasR、凋亡抗原1（APO-1或APT）、分化簇95（CD95）或肿瘤坏死因子受体超家族成员6（TNFRSF6），是一种由FAS基因编码的蛋白质。FAS受体是细胞表面的一种死亡受体，如果它与它的配体Fas配体（FasL）结合，会导致细胞程序性死亡（凋亡）。它是两种细胞凋亡途径之一，另一种是线粒体途径。最近，FAS也被证明可以促进肿瘤的生长，因为在肿瘤的发展过程中，它经常被下调或细胞对凋亡产生抵抗。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人凋亡相关因子(FAS;CD95)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人凋亡相关因子(FAS;CD95)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FAS;CD95. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FAS;CD95 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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