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几丁质酶1(CHIT1)又称为甲壳素酶1，是一个蛋白质编码基因。CHIT1是由活化的人类巨噬细胞分泌的，在戈谢病患者的血浆中明显升高。壳三糖苷酶的表达只发生在培养中单核细胞向活化巨噬细胞分化的晚期阶段。人类巨噬细胞可以合成一种功能性的壳核苷酸酶，这是一种高度保守的酶，其表达受到强烈调节。这种酶可能在降解含甲壳素的病原体中起作用。与CHIT1相关的疾病包括Chitotriosidase Deficiency和Gaucher's Disease。它的相关途径有：先天免疫系统和糖胺聚糖代谢。与该基因相关的基因本体论注释包括水解酶活性、水解O-糖基化合物和几丁质酶活性。该基因的一个重要类似物是CHIA。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人几丁质酶1(CHIT1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人几丁质酶1(CHIT1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CHIT1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CHIT1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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