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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
前列腺素还原酶2,也被称为PTGR2,是由PTGR2基因编码的酶。该基因编码一种参与前列腺素代谢的酶。编码的蛋白质催化15-酮前列腺素E2的NADPH依赖性转化为15-酮-13,14-二氢前列腺素E2。这种蛋白质也可能参与调节过氧化物酶体增殖物激活受体的激活。它作为15-氧代前列腺素13-还原酶起作用,作用于15-酮-PGE1,15-酮-PGE2,15-酮-PGE1-α和15-酮-PGE2-α,对15-酮-PGE2具有最高的活性。它的过表达抑制PPARG的转录活性并抑制脂肪细胞分化。替代拼接导致多个转录本变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人前列腺素还原酶2(PTGR2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人前列腺素还原酶2(PTGR2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PTGR2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PTGR2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.