计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
前蛋白转化酶枯草溶菌素2(PCSK2),也被称为原激素转化酶2或神经内分泌转化酶2(NEC2),是由PCSK2基因编码的酶。它是一种丝氨酸蛋白酶,与原蛋白转化酶1一样,PCSK2是负责许多神经内分泌肽从其前体成熟第一步的酶,例如将胰岛素原转化为胰岛素中间体。为了产生生物活性形式的胰岛素,需要涉及除去C末端碱性残基的第二步骤;该步骤由羧肽酶E和/或D介导。它与名为7B2的神经内分泌蛋白结合,如果该蛋白不存在,proPC2就不能变得酶活性。7B2通过防止proPC2聚集到不可灭活的形式来实现这一点。7B2的C端结构域也抑制PC2活性,直到它被切割成缺乏羧基末端碱性残基的较小非活性形式。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人前蛋白转化酶枯草溶菌素2(PCSK2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人前蛋白转化酶枯草溶菌素2(PCSK2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PCSK2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PCSK2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.