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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
半乳糖凝集素3结合蛋白，由LGALS3BP基因编码。银胶蛋白是一个β-半乳糖结合蛋白家族，与调节细胞-细胞和细胞-基质相互作用有关。LGALS3BP已被发现在癌症患者和人类免疫缺陷病毒（HIV）感染者的血清中升高。它似乎与自然杀伤（NK）和淋巴因子激活的杀伤（LAK）细胞的细胞毒性有关的免疫反应有关。利用荧光原位杂交技术，全长90K的cDNA已被定位到17q25号染色体上。与LGALS3BP相关的疾病包括Henoch-Schoenlein Purpura和Varicose Veins。其相关途径包括对血小板细胞膜Ca2+升高的反应。与该基因相关的基因本体论注释包括清道夫受体活性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人半乳糖凝集素3结合蛋白(LGALS3BP;M2BP)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人半乳糖凝集素3结合蛋白(LGALS3BP;M2BP)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LGALS3BP;M2BP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LGALS3BP;M2BP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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