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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
半乳糖凝集素9(Gal-9,Galectin-9)是从胚胎肾脏中分离出36 kDa的β-半乳糖凝集素蛋白。它由LGALS9基因编码。该蛋白有N端和C端碳水化合物结合结构域,由一个连接肽连接。已发现该基因有多种交替剪接的转录变体。Galectin-9是研究最多的HAVCR2(TIM-3)配体之一,在各种肿瘤细胞上表达。它也可以与其他蛋白(CLEC7A,CD137,CD40)相互作用。它还具有重要的细胞质、细胞内功能,并控制AMPK以应对溶酶体损伤,这种损伤在暴露于内源性和外源性膜损伤剂时可能发生,如晶体硅、胆固醇晶体、微生物毒素、蛋白病聚集物,如tau纤维和淀粉样蛋白,以及诱导溶酶体通透的信号通路。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人半乳糖凝集素9(GAL-9)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人半乳糖凝集素9(GAL-9)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GAL-9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GAL-9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.