计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-半乳糖苷酶,也称为乳糖酶、β-半乳糖苷,是一种糖苷水解酶家族,通过 打破糖苷键催化β-半乳糖苷水解为单糖。β-半乳糖苷包括含有半乳糖的碳水化合物,其中糖苷键位于半乳糖分子的上方。不同的β-半乳糖苷酶的底物包括神经节苷脂 GM1、乳糖甘油酰胺、乳糖和各种糖蛋白。β-半乳糖苷酶是一 种外糖苷酶,可以水解半乳糖和其有机基团之间形成的β-糖苷键。它也可以裂解岩藻糖和阿拉伯糖,但效率低得多。它是身体中的一种必需酶。该蛋白的缺陷可导致半乳糖醛酸症或莫基奥 B 综合征。在大肠杆菌中,lacZ基因是 β-半乳糖苷酶的结构基因;它作为诱导系统lac操作子的一部分存在,当葡萄糖水平低时,在乳糖存在下被激活。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人半乳糖苷酶β(GLb)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人半乳糖苷酶β(GLb)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GLb. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GLb present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.