BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
趋化因子配体2（CCL2）也被称为单核细胞趋化蛋白1（MCP1）和小诱导细胞因子A2。CCL2是一种小型细胞因子，属于CC趋化因子家族。它将单核细胞、记忆T细胞和树突状细胞招募到组织损伤或感染产生的炎症部位。CCL2和其他许多CC趋化因子位于17号染色体，基因跨度为1,927个碱基，它的基因有三个外显子和两个内含子。CCL2蛋白前体含有一个23个氨基酸的信号肽。反过来，成熟的CCL2有76个氨基酸。CCL2的预测重量是11.025千道尔顿（kDa）。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人单核细胞趋化蛋白1(MCP-1;CCL2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人单核细胞趋化蛋白1(MCP-1;CCL2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MCP-1;CCL2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MCP-1;CCL2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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